PONCEAU S FOR ELECTROPHORESIS
Ponceau S is a anionic dye commonly used for the detection of electro- blotted proteins after an electrophoretic separation prior to Western blotting [12–14] or MALDI-MS analysis [15].Ponceau S Staining Solution is supplied as ready to use. This product is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. This staining technique is often utilized to confirm protein electrotransfer in Western blotting assays prior to antibody-based detection.
Ponceau S is a rapid and reversible stain for detecting protein bands on Western blot membranes and can be used with PVDF, nitrocellulose and cellulose acetate membranes*.
Dosage
Place the blot transfer membrane in a plastic box and rinse it with water three times, 5 minutes each. Stain the membrane with Ponceau S stain for 30 seconds to 1 minute. Destain the membrane with several changes of water for 30 seconds to 1 minute each, then air dry