FIELD STAIN A FOR MICROSCOPY
Field Stains contain methylene blue and eosin. These basic and acidic dyes induce multiple colours when applied to cells. The fixative, methanol does not allow any futher change in slide. The basic component of white cells (cytoplasm) is stained by acidic dye and they are described as eosinophilic or acidophilic.
Field stain is a histological method for staining of blood smears. It is used for staining thick blood films to discover malarial parasites. Field's stain is a version of a Romanowsky stain, used for rapid processing of the specimens.
Dosage
ield's stain was applied by dipping the slide into Field's stain A for 3 seconds, then into tap water for 3 seconds (with gentle agitation), into Field's stain B for a further 3 seconds and then washing gently in tap water to remove excess stain.