TURKS SOLUTION
Türk's solution is a hematological stain (crystal violet or aqueous methylene blue) in 1-2% acetic acid and distilled water. ... Turk's solution is intended for use in counting leukocytes in a defined volume of blood. Erythrocytes are hemolyzed while leukocytes are stained for easy visualization.
In this protocol we describe how to visualize neutrophil extracellular traps (NETs) and fungal cell wall changes in the context of the coculture of mouse neutrophils with fungal hyphae of Candida albicans. These protocols are easily adjusted to test a wide array of hypotheses related to the impact of immune cells on fungi and the cell wall, making them promising tools for exploring host-pathogen interactions during fungal infection.
Türk's solution is a hematological stain (crystal violet or aqueous methylene blue) in 1-2% acetic acid and distilled water. The solution destroys the RBCs and platelets within a blood sample, and stains the nuclei of the white blood cells, making them easier to see and count.
Turk's solution is intended for use in counting leukocytes in a defined volume of blood. Erythrocytes are hemolyzed while leukocytes are stained for easy visualization
AQ treatment for 14 days at varying doses of 5 mg/kgBW
