TAPS BUFFER FOR BIOCHEMISTRY
TAPS ([tris(hydroxymethyl)methylamino]propanesulfonic acid) is a chemical compound commonly used to make buffer solutions.
Store away from strong oxidizing agents. Keep container tightly closed. Store in cool, dry conditions in well sealed containers.
TAPS, is used in biochemistry and molecular biology. TAPS has been utilized in capillary electrophoresis of such materials as DNA and DNA-dye complexes. Dyes have been separated by planar chromatography with electro osmotic flow with 1 mM TAPS in the mobile phase. The stability constants of metal ions with TAPS have been investigated by capillary electrophoresis. TAPS has also been shown to inhibit connexin channel activity. The activity of various mutants of carbonic anhydrase I and carbonic anhydrase II has been studied in a variety of buffers, including TAPS.
