R.B.C. Diluting Fluid (Grower's) is used as diluting fluid for blood specimen to count the red blood cells under high powder by heamocytometry. In Vitro diagnostic use only.
Note
best RBC diluting fluid. ...
Formalin acts as the preservative; cell shape is not altered. ...
not recommended because it allows growth of yeasts. ...
produces clumping of cells particularly with blood of patients suffering from liver cirrhosis. ...
It can stand for a long period of time and has no corrosive effect.
Gower's Fluid (RBC) superior to Hayem's fluid. prevents ROULEAUX FORMATION and PRECIPITATION OF PROTEINS in cases of HYPERGLOBULINEMIA and HEMOGLOBULINEMIA.
RBC diluting fluid is isotonic with blood, hence hemolysis does not take place. Normal Saline also can be used. But it causes slight creation of red blood cells and allows rouleaux formation.